![]() Later studies revealed sulfated polysaccharides from marine algae have many biological and physiological activities including anticoagulant, antithrombotic, anti-inflammatory, antiviral, and activities. Marine algal cell walls were reported to contain sulfated polysaccharides, which are not found in land plants and may have specific functions in ionic regulation. In the search for novel compounds with antitumor properties, marine bioresources have become particular interest given their unique bioactivities. The biological activities of these polysaccharides have attracted considerable attention in the biotechnology and medical fields. ![]() Many polysaccharides have been isolated from mushrooms, fungi, yeast, algae, lichens and plants in the search for potential anticancer drugs. This effect of carrageenan towards cancer cells could possibly be developed into a tumor cell-specific anticancer agent.Ĭancer is the leading cause of death worldwide, accounting for more 8.2 million deaths in recent years. Additionally, different types of carrageenans have different effects on cell cycle progression. ConclusionĬonsidering these results, it could be suggested that carrageenan affects the cell-cycle of HeLa cells not only by arresting the cell cycle in specific phases but also by delaying the time needed for the cell to progress through the cell cycle. Furthermore, according to cell-cycle analysis with FUCCI cells, the cell cycle of HeLa cells was delayed in specific phases due to different carrageenan treatments. In contrast both forms of carrageenan were cytotoxic towards HeLa cells (cancer cells). ResultsĬarrageenan had no significant effect on HUVEC (normal cells). Cell-cycle profile and progression were demonstrated with HeLa cells expressing FUCCI (fluorescence ubiquitination-based cell-cycle indicator) probes (HeLa-FUCCI). Cell viability was determined using the MTT assay while cell death rates were determined using staining with calcein-AM/propidium iodide. Using human cervical carcinoma cells (HeLa) cells as and human umbilical vein endothelial cells (HUVEC), the cytotoxic effects of kappa carrageenan (k-CO) and lambda carrageenan (λ-CO) at the concentrations of 250–2500 μg/mL were observed. Thus, in this study we investigated the effects of carrageenan on the tumor cell cycle. Recently there is an increasing interest in potential anticancer agents that affect cell cycle in cancer cells. One of the hallmarks of cancer is uncontrolled proliferation, a consequence of loss of normal cell-cycle control, that underlies tumor growth. ![]() Previous findings show various effects of carrageenan suppressing tumor cell growth. Have a listen:Īs for Dead Cells itself, it's currently 40% off as a part of the Steam Summer Sale, so if you ever wanted to give it a try, this would be a good time to do so.Carrageenan is a sulfated polysaccharide that exists in red seaweeds recently shown to have anticancer properties. If you prefer the short version, however, you can learn a little bit more about the update through the newest developer vlog. You can find all of the details, including the full patch notes, over at Dead Cells website. The "Update of Plenty" has also reworked the merchant and in-game economy in order to make things a bit more intuitive, as well as greatly adjusted crossbows and how often scrolls appear in early biomes. So while these changes make things more reasonable and approachable for beginners, veteran players should still find plenty of challenging content to mess around with. Under the new system, 0BC has been made slightly more challenging in order to teach new players the ropes, while the difficulty curve between 0-5BC has been adjusted to remove any massive spikes in difficulty. The definitive highlight is the smoothed out difficulty progression that should make it so new players don't just hit a wall at 2BC (two Boss Cells) and then proceed to die over and over and over again until they quit. In an effort to improve its replayability even further, as well as make things a bit easier for beginners, Dead Cells developers have now released a rather extensive balance update. : Dead Cells' second DLC, Fatal Falls, is set to launch this January 26th.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |